CD94 is a 27-31kD glycoprotein member of the C-type lectin superfamily.
It is expressed on NK cells, particularly those that are CD56bright, plus those that are CD56dim but secrete material amounts of IFN-G.
CD94 forms covalent transmembrane (TM) heterodimers with NKG2A, B, C, E and H, and binds HLA-E (in human; Qa-1b in mouse) as part of a cellular homeostatic monitoring system.
Amide-linkage based complexes with NKG2A are predicted to run at 70-80kD in SDS-PAGE.
Depending upon the exact NKG2 partner, CD94 ligation can result in either NK cell activation (2C, 2H or 2E) or inhibition (2A or 2B).
Human CD94 is a 179aa type II TM protein.
It possesses a short cytoplasmic segment aa1-10 plus a 158aa extracellular region aa32-179 that contains one C-type lectin domain aa61-176.
Multiple splice variants exist.
One is a 149aa, 19kD cytosolic protein that shows an Ala substitution for aa3-34, while a second is a truncated, 104aa, 17kD TM protein.
A third isoform is 158aa in length and 25kD in size, and shows a deletion of aa34-54.
Over aa32-179, human CD94 shares 53% aa identity with mouse CD94.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human CD94 (native and recombinant). Species Crossreactivity: mouse and canine.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Ca Hu Mo
Immunogen：Recombinant corresponding to aa32-179 from human CD94 expressed in CHO cell line.
