Caspase-10 is a 28-29kD member of the peptidase C14A family of enzymes.
It is widely expressed, being found in blood and epithelial cells.
Caspase-10 appears to be an initiator caspase that acts on procaspases-3, 4, 6, 7, and 9.
Human procaspase-10a is a 59kD, 521aa protein.
It contains two N-terminal death domains.
Cleavage generates p43 plus the small mature p12 subunit.
Cleavage of p43 generates a prodomain p25 and large mature p17 subunit.
P17 and p12 noncovalently associate to form a 29kD mature heterodimer.
There are at least four isoform variants.
There is no apparent mouse Casp-10 gene.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunocytochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human Pro Caspase-10. Species sequence homology: Recombinant human Caspase-2, rhCaspase-3 (long and short forms), rhCaspase-3, rhCaspase-7, rhCaspase-8, rhCaspase-8 p10, rhCaspase-8 p20 and rhCaspase-9 (1%).
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa1-228 from human Pro Caspase-10 expressed in E.coli.
