84-1215-59　Brain Natriuretic Peptide Pro (proBNP, Natriuretic Peptides B, Gamma-brain Natriuretic Peptide, NPPB) 100ug　B2702-01N

特徴

• Human BNP is a 3.5kD, secreted, nonglycosylated peptide that belongs to the natriuretic peptide family.
• It is the product of ventricular myocytes and bone marrow endothelial cells.
• BNP is synthesized as a 12kD, 108aa prohormone with a 76aa prosegment and a 32aa mature (BNP) region.
• Both forms circulate, with the prohormone showing O-linked glycosylation that generates a 25-27kD glycoprotein.
• Glycosylation is suggested to stabilize the molecule.
• The proform also tends to noncovalently trimerize.
• In blood, 1-32 BNP is proteolytically processed into 3-32 BNP, with loss of Ser-Pro at the N-terminus.
• Human Pro-BNP is 29% and 50% aa identical to mouse and pig Pro-BNP.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.