BMP-8b is a 16kD predicted mature and 45kD precursor member of the Gbb-60A class of the BMP family of molecules.
In mouse, precursors to sperm and oocytes arise from the proximal epiblast around day E7.5 (days 18-20 in human).
This lineage commitment is a response to BMP-4 signaling, which is promoted by the repressive action of BMP-8b on the anterior visceral endoderm, a source of BMP-4 antagonism.
Mouse BMP-8b preproprecursor is 399aa in length.
It contains a 19aa signal sequence, a 241aa prosegment, and a 139aa mature region.
An interchain disulfide bond occurs at 363aa, generating a homodimer.
Over aa261-399, mouse BMP-8b shares 74% aa identity with mouse BMP-8a, and 69% and 93% aa identity with human and rat BMP-8b.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes mouse BMP-8b. Species sequence homology: Recombinant human BMP-3b, rhBMP-10, and recombinant mouse BMP-10 human (10%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Recombinant corresponding to aa261-399 from mouse BMP-8b expressed in E.coli.
