84-1215-53　Angiotensinogen (Serpin A8, AGT, SERPINA8) 100ug　A2294-48S

特徴

• Serpin A8 is a secreted, 52-62kD glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors.
• It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten aa peptide termed Ang/angiotensin I.
• This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II and III.
• Ang II induces vascoconstriction and aldosterone release by acting on AT1 receptors, while Ang III drives aldosterone release.
• Ang I can be further processed by MME to generate Ang, a peptide that binds MAS1 on platelets, and promotes the release of NO, an antithrombotic agent.
• Mature mouse angiotensinogen is 453aa in length aa25-477.
• It contains Ang I aa25-34 that is cleaved to create Ang II aa25-32, Ang III aa26-32 and Ang I aa25-31.
• Serpin A8/Angiotensinogen may circulate in a 200kD complex with major basic protein (MBP), or as part of a larger 300kD complex with MBP and complement C3dg.
• There is an alternative start site 5aa upstream of the standard site.
• Over aa25-477, mouse serpin A8 shares 86% and 61% aa identity with rat and human serpin A8.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.