A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexins, through which materials of low molecular weight diffuse from one cell to a neighboring cell.
Each connexin is composed of a hexamer of connexin proteins.
Connexins are a multi-gene family of highly related proteins.
At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner.
Two distinct lineages, class I (beta) and class II (alpha), have been identified in mammals.
Connexin-43, also known as Gap Junction Alpha-1 (GJA1), belongs to the class II (alpha) group of connexins and is expressed in the heart and fetal cochlea.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 0.5-1ug/ml Immunohistochemistry (formalin fixed and paraffin): 1-2ug/ml.
Antigen retrieval is required
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Dilute in PBS (pH 7.4).
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS pH 7.4, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute in 100ul of sterile ddH2O.
Specificity：Recognizes Connexin-43. Species crossreactivity: human, mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to the amino acids 366-382 from the C-terminus of human Connexin-43.
