Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:200-1:5000Western Blot: 1:200-1:5000Immunohistochemistry: 1:500-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months at -20°C after receipt.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:1mg
Host:sheep
Source Antibody:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free, 0.09% Kathon CG. Reconstitute with 1.1ml sterile ddH2O. Labeled with horseradish peroxidase (HRP).