Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:200-1:5000Western Blot: 1:200-1:5000Immunohistochemistry: 1:500-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
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Note: Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA (Protease and IgG free), 0.1% Kathon CG. Labeled with horseradish peroxidase (HRP). Reconstitute with 1.1ml sterile ddH2O.
Specificity：Recognizes heavy chain of human IgE. No reactivity has been observed with non-IgE human serum immunogloublins and with light chains on all human immunoglobulins. Does not react with serum proteins from bovine, mouse or rabbit
