Biotinylated Anti-Avidin D is an unusual molecule.
It is capable of binding to Avidin D by two mechanisms, either through the antigen binding sites in the Fab portion of the antibody or through multiple biotin residues covalently attached to the protein.
Although little is known about the mechanism of binding, this reagent appears to provide superior amplification of fluorescent signals compared to biotinylated "extender" molecules or anti-avidin alone.
The steps involved in using this product are straightforward.
After a biotinylated antibody has been introduced, cells or sections are labeled with Fluorescein Avidin DCS, followed by Biotinlyated Anti-Avidin D, and then a second labeling with Fluorescein Avidin DCS.
This procedure results in the introduction of several more fluorochromes at the antigenic site in the tissue.
Cell sorter analysis has shown that a several-fold increase in fluorescence can be achieved in some antibody labeling studies using Biotinylated Anti-Avidin D.
Other applications of this unique reagent are currently under investigation.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 1-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：goat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with Biotin.
