The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell.
The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif.
This motif is composed of four amino acids at the C-terminal end of the protein sequence.
The most common retention sequence is KDEL (lys-asp-glu-leu).
Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence.
The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
Applications:Suitable for use Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 detects KDEL-containing proteins in 20ug of HeLa cell lysateOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes KDEL proteins Grp94, Grp78, PDI and Calreticulin. It may also react with ERp57 and ERp29.
