SMADs are proteins that modulate the activity of transforming growth factor beta ligands.
The SMADs,often in complex with other SMADs/CoSMAD, act as transcription factors that regulate the expression of certain genes.
Zhu, H et al concluded that targeted ubiquitination of SMADs may serve to control both embryonic development and a wide variety of cellular responses to TGF-beta signals.
R-Smads or receptor regulated Smads are a class of proteins that include SMAD1, SMAD2, SMAD3, SMAD5, and SMAD8.
In response to signals by the TGF-b superfamily of ligands these proteins associate with receptor kinases and are phosphorylated at an SSXS motif at their extreme C-terminus.
These proteins then typically bind to the common mediator Smad or co-SMAD SMAD4.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 5mg BSA, 0.9mg sodium chloride, 0.2mg disodium hydrogen phosphate, 0.05mg thimerosal, 0.05mg sodium azide.
Specificity：Recognizes human Smad 1,2,3,5. Species Crossreactivity: Rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa442-456 from human Smad 1,2,3,5 at C-terminal.
Shelf Life：1year
EU Commodity Code：30021010
商品名：Smad1, Smad2, Smad3, Smad5 (Mothers Against Decapentaplegic Homolog 1, MAD Homolog 1, Mothers Against DPP Homolog 1, JV4-1, Mad-related Protein 1, SMAD Family Member 1, SMAD 1, Transforming Growth Factor-beta-signaling Protein 1, BSP-1, BSP1, MADH1, MADR1, Mothers Against Decapentaplegic Homolog 2, MAD Homolog 2, Mothers Against DPP Homolog 2, JV18-1, Mad-related Protein 2, hMAD-2, SMAD Family Member 2, SMAD 2, MADH2, MADR2, Mothers Against Decapentaplegic Homolog 3, MAD Homolog 3, Mad3, Mothers Against DPP Homolog 3, hMAD-3, JV15-2, SMAD Family Member 3, SMAD 3, hSMAD3, MADH3, Mothers Against Decapentaplegic Homolog 5, MAD Homolog 5, Mothers Against DPP Homolog 5, JV5-1, SMAD Family Member 5, SMAD 5, hSmad5, MADH5) 100ug
