84-1213-41　NOS1 (Nitric Oxide Synthase, Brain, Constitutive NOS, NC-NOS, NOS Type I, Neuronal NOS, N-NOS, nNOS, bNOS, Peptidyl-cysteine S-nitrosylase NOS1) 100ug　N5350-03L

特徴

• Nitric Oxide Synthase 1(NOS1, neuronal NOS, nNOS1) is a messenger molecule, mediating the effect ofendothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain.
• It may be involved in neuronal cell death and damage in neurological illness.
• nNOS1 localized to the 12q24.2 region of human chromosome 12.
• It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098aa.
• nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2.Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300bp of each other but separated from exon 2 by an intron that is at least 20kb in length.
• The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle.
• The novel gaseous neuromediator nitric oxide is thought to play an important role in development and plasticity.
• Despite this, gene-knockout mice lacking neuronal (Type I) nitric oxide synthase exhibit relatively normal brain development and behavior.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.