84-1213-20　NFkB, p100/p52 (Nuclear Factor NF-kappa-B p100 Subunit, DNA-binding Factor KBF2, H2TF1, Lymphocyte Translocation Chromosome 10 Protein, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B Cells 2, Oncogene Lyt-10, Lyt10, Nuclear Factor NF-kappa-B p52 Subunit, NFKB2, LYT10) 100ul　N2305-02K

特徴

• NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer.
• Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2).
• The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types.
• Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells.
• Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini.
• Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts.
• The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.
• Applications:Suitable for use in Immunofluorescence, Immunocytochemistry, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunofluorescence (IF-IC): 1:100-1:200Immunofluorescence 1:100-1:200Western Blot: 1:500-1:1000Immunohistochemistry (paraffin): 1:50-1:100Optimal dilutions to be determined by the researcher.
• Positive Control: NFkB p105/p50 Overexpression Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.