SOX-9 is a member of the family of SOX (Sry-type high mobility group box) gene that were first identified on the basis of region with high homology to that of Sry (Sex-determining region Y).
It is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen.
Phosphorylation of SOX-9 by PKA increases its DNA binding and transcriptional activity.
SOX-9 regulates expression of chondrocyte phenotype related genes.
IL-1 and TNF-a can cause a marked and rapid decrease in the levels of SOX9 mRNA and/or protein in chondrocytes, thus strongly inhibit the expression of genes for cartilage extracellular matrix proteins.
The down-regulation of SOX-9 may have a crucial role in inhibiting expression of the cartilage phenotype in inflammatory joint diseases.
Applications:Suitable for use in Western Blot and Dot Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlDot Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity：Recognizes human SOX-9, phosphorylated at Serine 181.
Isotype：IgG
Calc Applications Abbrev：DB WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human SOX-9, phosphorylated at Serine 181 (RRKpSVKN).
