The retinoblastoma-associated protein (Rb1) is one of the key cell-cycle regulating proteins that act as a tumor suppressor and its inactivation leads to neoplastic transformation and carcinogenesis.
This protein regulates critical G1-to-S phase transition through interaction with the E2F family of cell-cycle transcription factors repressing transcription of genes required for this cell-cycle checkpoint transition.
Its activity is regulated through network sensing intracellular and extracellular signals that block or permit phosphorylation (inactivation) of the Rb protein.
It also regulates apoptosis through the same interaction with E2F transcription factors and that Rb–E2F complexes play a role in regulating the transcription of genes involved in differentiation and development.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000-1:20,000Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Control Peptide: R1660-05V
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Source Antibody：Zebrafish
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes zebrafish RB-1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ze
Immunogen：Synthetic peptide corresponding to the intermediate region of zebrafish RB-1 (Accession# NP_001071248).
