84-1212-33　［取扱停止］p53, Phosphorylated (Ser18/23) (Cellular Tumor Antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor Suppressor p53, TP53) 50ug　P1000-70C

特徴

• The p53 tumor suppressor is a transcription factor that functions to regulate cell proliferation and apoptosis.
• The important of p53 in suppressing cancer is highlighted by the very high incidence of spontaneous tumor formation in p53-deficient mice and in mice bearing genetic alteration in other members of the p53 pathway.
• P53 becomes stabilized and activated in response to inverse cellualar stressed such as DNA damage, hypoxia, ribonucleotide depletion and oncogene activation.
• Phosphorylation occurs in response to DNA damage on human p53 serine residues 15 and 20 (mouse Ser18 and Ser23, respectively) and has been found in vitro studies to upregulate p53 activity.
• Phosphorylation of serine 18 was required for robust p53-mediated apoptosis but not required for p53-mediated tumor suppression.
• Phosphorylation at Ser20 (murine Ser23) of the p53 tumor suppressor is critical for disrupting p53 intraction with its negative regulator, MDM2, and allowing p53 stabilization.
• Serine 23 phosphorylation in p53 plays an important role in p53 reponse to DNA damage in vivo and in tumor suppression.
• Applications:Suitable for use in ELISA, Western Blot and Dot Blot.
• Other applications not tested.
• Recommended Dilutions:ELISA: 0.1-1ug/mlWestern Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.