TCF4 (T cell factor 4; also known as TF7L2) serves as transcriptional activator affecting e.g.
cell cycle and terminal cell differentiation.
In the absence of Wnt signals, TCF4 interacts with TLE/Groucho corepressor, supressing the transcription of TCF4 target genes.
Wnt signaling leads to stabilisation and accumulation of beta-catenin, which binds TCF4 N-terminus, whereas TCF4 C-terminal HMG-box domains interact with DNA.
Cell death-associated Daxx protein or plakoglobin belong to negative regulators of TCF4-mediated transcription, on the other hand, there is a positive feedback loop of beta-catenin-TCF4 pathway comprising the p300 coactivator.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml Immunocytochemistry: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 15mM sodium azide, pH 7.4.
Specificity：Recognizes human TCF7L2. Species sequence homology: Mouse.
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to bacterially expressed full-length human TCF4.
