Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals.
They are also present, at lower levels, in various other eukaryotic tissues.
14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival.
As 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases.
14-3-3 sigma may be required for Raf-1 kinase activity and phosphorylation, among many other functions.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 1:250Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：sheep
Grade：Serum
Purity：Serum
Form：Supplied as a liquid , 0.09% sodium azide.
Specificity：Recognizes 14-3-3 sigma in all mammals. Species sequence homology: Bovine, chicken, human, mammal, mouse, rabbit, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Sh
Immunogen：Synthetic peptide MERASLIQKAC corresponding to acetylated N-terminal sequence of sheep 14-3-3 sigma.
