Oct4 (Octamer binding transcription factor 4) is an important marker of the undifferentiated state and a central regulator of pluripotency in ES cells.
When embryonic stem cells are triggered to differentiate, Oct-4 is downregulated thus providing a model for the early events linked to somatic differentiation in the developing embryo.
Oct4 is nucleus protein, with a predicted molecular weight 39kD.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200Western Blot: 1:500-1:3000Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml Immunocytochemistry: 1:100-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.0, 0.01% thimerosal, 10% glycerol.
Specificity：Recognizes human OCT3/4. Species sequence homology: Rabbit (85%), bovine (85%), porcine (88%), monkey (97%).
Isotype：IgG
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to human OCT3/4.
