84-1211-18　NOS1 (Nitric Oxide Synthase, Brain, Constitutive NOS, NC-NOS, NOS Type I, Neuronal NOS, N-NOS, nNOS, bNOS) 50ug　N5350-01U

特徴

• Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells.
• Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO.
• NO production is mediated by members of the nitric oxide synthase (NOS) family.
• NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO.
• Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned.
• All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
• bNOS and eNOS share approximately 50% sequence homology and their enzymatic activity depends on binding to the calcium/calmodulin complex.
• Increases in intracellular calcium lead to the production of low levels of NO over a short time.
• bNOS is found in neurons, peripheral nerve cells, macula densa, and pancreatic islet cells.
• Alternate splicing specifically regulates bNOS in striated muscle.
• The translated protein, bNOS mu, is 34aa residues larger than bNOS from brain.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:250Immunohistochemistry (Formalin fixed paraffin embedded): 20ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.