Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism.
Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export.
GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease.
GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200Western Blot: 1:5000-1:20,000Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlImmunocytochemistry: 1:100-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.0, 1% BSA, 0.01% thimerosal, 20% glycerol.
Specificity：Recognizes human GAPDH. Species sequence homology: Feline, bovine, canine, mouse, rabbit, rat, zebrafish.
Isotype：IgG
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa9-323 of human GAPDH.
