This protein regulates eIF4E activity by preventing its assembly into the eIF4F complex.
Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway.
Nonphosphorylated EIF4EBP2 interacts with EIF4E.
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF.
Belongs to the eIF4E binding protein family.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 4ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 25mM sodium borate, 100mM boric acid, 75mM sodium chloride, 5mM EDTA, pH 8.4.
Specificity：Recognizes human EIF4EBP2.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa99-120 of human PHAS-II conjugated to KLH. Species sequence homology: Bovine, canine, mouse, porcine, rat.
