Vascular adhesion protein 1 (VAP-1), also known as AOC3 (amine oxidase copper-containing 3) or SSAO (semicarbazide-sensitive amine oxidase) is a 170kD type II transmembrane glycoprotein consisting of two disulfide-linked 763aa subunits.
It is expressed on endothelium, vascular smooth muscle, and adipocytes.
The extracellular domain mediates lymphocyte rolling, adhesion, possibly extravasations during inflammation, and possesses monoamine oxidase activity.
Adipocytes can release a soluble form via metalloproteinase cleavage.
Human VAP-1 ECD shares 83% and 88% aa sequence identity with mouse and canine VAP-1 ECD, respectively.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:64,000Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3.75ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human AOC3. Species sequence homology: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-DEDPSFYSADSIY from an internal region of human AOC3.
