84-1209-66　［取扱停止］USP18 (Ubl Carboxyl-terminal Hydrolase 18, 43kD ISG15-specific Protease, hUBP43, ISG15-specific-processing Protease, Ubl Thiolesterase 18, ISG43) 50ug　U4100-85B

特徴

• Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action.
• Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD and JAMM enzymes.
• USP18 (also known as UBP43) is a deubiquitinase best known for catalyzing the removal of ISG15, an interferon-regulated ubibiquitin-like protein, from conjugated proteins.
• Removal of ISG15 from target proteins by the USP18 peptidase maintains the critical cellular balance of ISG15-conjugated proteins important for normal development and brain function.
• Following induction by IFN or LPS, USP18 binds the INF receptor subunit IFNAR2 and inhibits signal transduction through the JAK-STAT pathway.
• USP18 regulation of IFN signaling inhibits IFN-mediated apoptosis and does not necessarily rely on USP18 peptidase activity.
• As the therapeutic use of recombinant IFN can lead to refractory IFN signaling and a less effective response, the combination of IFN treatment and regulation of USP18 expression may produce a more positive outcome.
• Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 1:500-1:2000Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.