Ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1), also known as PGP 9.5, is a neuronal and neuroendocrine-specific protein that plays a role in proteosomal protein degradation, which is critical for neuronal health.
UCH-L1 is also involved in p27kip1 degradation through its interaction and nuclear translocation with JAB1.
UCH-L1 expression is specifically associated with non small-cell lung cancer development and it is found in serum of patients with lung cancer, indicating that it may be important in lung cancer screening and diagnosis.
UCH-L1 may also be a useful marker for invasive colorectal cancer.
Mutations in the alpha-synuclein and Uch-L1 genes cause autosomal dominant Parkinson's disease (PD).
Derangements in the ubiquitin proteasomal pathway may play important roles in the demise of dopamine neurons in PD.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes human UCHL1.
Isotype：IgY
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa103-160 of human UCHL1.
