84-1208-80　［取扱停止］SIRT2, CT (SIRT2, NAD-dependent Deacetylase Sirtuin-2, SIR2-like Protein 2, SIR2L, SIR2L2) 50ug　S1013-88D6

特徴

• Sir2 belongs to a family of proteins that is found in organisms ranging from bacteria to complex eukaryotes.
• Members of this family contain a 250 aa core domain that shares about 25-60% sequence identity.
• Silencing occurs as a series of events initiated by formation of Sir complexes (Sir2, Sir3, Sir4).
• The complexes are recruited to their chromosome targets via interactions with DNA-binding proteins, followed by deacetylation of histones H3 and H4.
• A final step required for telomeric silencing is binding of the complex to the deacetylated histones and recruitment of the telosome to the nuclear periphery.6 Sir2 protein is an NAD-dependent histone deacetylase, an enzyme that removes acetyl groups from lysine residues of histone proteins and possibly other substrates.
• Sir2 transfers acetyl groups from its protein substrates to ADP-ribose and synthesizes o-acetyl-ADP-ribose.
• Through histone deacetylation, Sir2 may silence chromatin.
• It appears that Sir2 NAD requirement makes this protein an important player in the pathway that leads to increased life span of several species through calorie restriction.
• The maintenance or silencing of chromatin may be at the center of processes leading to aging of cells and development of cancer.
• Applications:Suitable for use in Western Blot, ELISA and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.