Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ.
The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane.
The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:8000Western Blot: 0.03-0.1ug/mlImmunohistochemistry (Formalin-fixed, paraffin-embedded): 5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:Recognizes human SDHB.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to C-ATYKEKKASV from the C-terminal of human SDHB.