RAF, a member of the Raf Subfamily of Serine/Threonine Protein Kinases, is part of the RAS-dependent signalling pathway.
Once activated, Raf-1 can activate the dual specificity protein kinases MEK1 and MEK2 which in turn activate the serine/threonine specific protein kinases ERK1 and ERK2.
Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
t(3;8)(p25;q21) translocations involving RAF1 result in mixed parotid gland tumors.
Raf1 is an oncogene that has been reported to be overexpressed in many tumor types.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human RAF1. Species sequence homology: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic non-phosphopeptide corresponding to aa surrounding Ser259 of human RAF1 (KLH).
