PSMA (prostate specific membrane antigen) is a 100-120kD type II, integral membrane glycoprotein,1 composed of a 19aa intracellular domain containing the N-terminus, a 24aa transmembrane region, and a 707aa extracellular C-terminal domain.
The PSMA gene has been cloned and sequenced, and has been localized to chromosome 11q.
Three functionally distinct proteins are encoded, including folylpoly-gamma-glutamate carboxypeptidase in the intestine, N-acetylated alpha-linked acidic dipeptidase 1 in the brain, and prostate-specific membrane antigen in the prostate.
PSMA is highly expressed in prostate secretory-acinar epithelium, in some benign extraprostatic epithelial cells from breast, duodenum, and kidney tissues, and in prostate cancer.
On the basis of its highly prostate-specific nature, PSMA has been used as a prostate cancer marker antigen and diagnostic target for prostate cancer detection.
Recently, evidence of limited and specific endothelial PSMA expression has been discovered: the neovasculature of a wide variety of malignant neoplasms (lung, colon, breast, etc.) demonstrates PSMA expression.
This finding suggests that PSMA may be an effective target for monoclonal antibody-based anti-neovasculature therapy.
PSMA has also been identified in the human nervous system.
Based on activity, immunoreactivity, and mRNA sequence comparison with one form of NAALADase, a neuropeptide that may modulate glutaminergic transmission in the nervous system, PSMA was found to be indistinguishable from NAALADase derived from human cerebellar cell isolates.
Therefore, the form of NAALADase also known as PSMA is expressed in brain, where it makes up a significant fraction of brain NAALADase activity.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin-fixed, paraffin-embedded): 15ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in10mM PBS, pH 7.4, BSA, sodium azide.
Specificity：Recognizes human PSMA.
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human PSMA.
