PLA2G16 (Group 16 phospholipase A2; also HREV107 and AdPLA) is a Ca++-dependent 17-18kD member of the H-rev107 family of molecules.
It is expressed intracellularly by white adipocytes, induced by insulin, and generates arachadonic acid from phospholipids.
The arachadonic acid is converted to PGE2, which binds the EP3 receptor and decreases cAMP levels.
This results in decreased hormone-sensitive lipase activity and increased adiposity.
Human PLA216 is 16211aa in length.
It is associated with both membranes and cytosol, and may possess a transmembrane segment between aa134-154.
The enzymatic activity resides in His23 and Cys113.
There is one potential isoform that shows a 20aa substitution for the first 4aa.
Over aa12-132, human PLA2G16 shares 88% aa identity with mouse PLA2G16.
Applications:Suitable for use in Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlELISA: 1:128,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human PLA2G16. Species sequence homology: Bovine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to RAPIPEPKPGDLIE from human PLA2G16 at N-terminal.
