This gene is a main control point for the regulation of gluconeogenesis.
The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP.
The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet.
Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency.
A mitochondrial isozyme of the encoded protein also has been characterized.
[provided by RefSeq]
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:8000Immunohistochemistry (Formalin fixed paraffin embedded): 2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:Recognizes human PCK1. Species sequence homology: Canine, mouse and rat.
Isotype:IgG
Calc Applications Abbrev:E IHC
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to C-HVNWFRKDKEGK from an internal region of human PCK1.