PCNA is found in the nucleus and is a cofactor of DNA polymerase delta.
This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication.
In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 2-4ug/mlImmunohistochemistry (Frozen,Formalin-fixed, paraffin-embedded): 1:100Immunohistochemistry: FrozenOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% thimerosal, 50% glycerol.
Specificity：Recognizes human PCNA.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the C-terminal of human PCNA. Species sequence homology: mouse and rat
