OCT4 is a mammalian POU transcription factor expressed by early embryo cells and germ cells.
The human OCT4 gene maps to chromosome 6 and has two isoforms with identical C-termini that include the characteristic POU-specific domain and the POU home domain.
OCT4 is initially expressed in unfertilized oocytes and then in totipotent and pluripotent cells of the inner cell mass of pre-gastrulation embryos before becoming specific to germ-line cells.
The OCT4 gene is expressed at low levels in such diverse adult tissues as heart, kidney, liver, spleen, and pancreatic islets.
Specific functions of OCT4 have not been established.
The expression of OCT4 in unfertilized oocytes and blastocysts is probably of greatest significance because of the role that OCT4 may be playing in early post fertilization events.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:62,500Western Blot: 1.25ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in PBS, 2% sucrose.
Specificity：Recognizes human POU5F1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human POU5F1 (KLH).
