84-1207-83　NOS2, CT (Nitric Oxide Synthase, Inducible, Hepatocyte NOS, HEP-NOS, Inducible NO Synthase, Inducible NOS, iNOS, NOS Type II, Peptidyl-cysteine S-nitrosylase NOS2, NOS2A) 50ug　N5350-10E

特徴

• Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells.
• Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO.
• NO production is mediated by members of the nitric oxide synthase (NOS) family.
• NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO.
• Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned.
• All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
• iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells.
• Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels.
• Protein kinase C-stimulating agents exhibit the same effect on iNOS activity.
• After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 1-2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.