Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells.
Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO.
NO production is mediated by members of the nitric oxide synthase (NOS) family.
NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO.
Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned.
All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells.
Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels.
Protein kinase C-stimulating agents exhibit the same effect on iNOS activity.
After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NOS1. Species sequence homology: Rat.
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa1422-1433, ESKKDTDEVFSS, from human NOS1 (KLH).
