84-1207-79　［取扱停止］NOD2, NT (CARD15, Nucleotide-binding Oligomerization Domain-containing Protein 2, Caspase Recruitment Domain-containing Protein 15, Inflammatory Bowel Disease Protein 1, IBD1) 50ug　N3006-14L

特徴

• NOD2 is an intracellular protein of 100kD, which belongs to the NOD (nucleotide binding oligomerisation domain) family.
• The NOD family comprises at present more than 20 different mammalian NOD-LRR (leucine-rich repeat) proteins, which are involved in the regulation of apoptosis and the immune response.
• NOD2 has been shown to be constitutively or inducibly expressed in monocytes, macrophages, T and B cells, dendritic cells, as well as intestinal epithelial cells.
• A specific motif of peptidoglycan (PGN), muramyl dipeptide (MDP), has been identified as the sole ligand of NOD2.
• NOD2 and Toll-like receptors (TLR) are emerging as key mediators of innate host defence in the intestinal mucosa, crucially involved in maintaining mucosal and commensal homeostasis.
• Mutations in the gene encoding NOD2 are a major susceptibility factor underlying a significant subgroup of patients with Crohn's disease.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 2-4ug/ml Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3ug/mlImmunocytochemistry: 5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.