NFKB1 or NFKB2 bind to REL, RELA, or RELB to form the NFKB complex.
The NFKB1/RELA heterodimer is the most abundant form of NFKB.
The NFKB complex is inactivated by I-kappa-B proteins that keep it trapped in the cytoplasm.
Phosphorylation of serine residues on the I-kappa B proteins mark them for ubiquitination and destruction, and NFKB translocates to the nucleus where it binds DNA.
Transforming protein Tax inhibits p53 transcriptional activity through the NFKB signaling pathway, specifically via the p65 (RelA) subunit.
The inhibition of p53 activity is dependent upon phosphorylation of p65 (RelA) at ser536 by the upstream kinase IKKB.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human RELA. Species sequence homology: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa surrounding Ser536 of human RELA (KLH).
