NALP3 is a cytoplasmic, pyrin-like protein belonging to the NLRP family with a DPIN domain, seven leucine rich repeats and a NACHT domain.
It is known to interact with apoptosis-associated speck-like protein PYCARD/ASC and is a part of the NALP3 inflammasome complex.
NALP3 is involved in the activation of caspase-1 and caspase-5 in response to a number of triggers including bacterial or viral infection, thus leading to the processing of IL-1B and IL-18.
It may also inhibit TNF-alpha-induced activation and nuclear translocation of RELA/NF-KB p65 and function in the activation of NF-kappa-B signaling, thus regulating inflammation and apoptosis.
Expression is predominantly in peripheral blood leukocytes, polymorphonuclear cells, osteoblasts and chondrocytes.
NALP3 defects leads to several diseases including familial cold auto inflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID).
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:64,000Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in TBS, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human NLRP3. Species sequence homology: Canine.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to internal region of human NLRP3 (KLH).
