MEK2, a MAP2K type protein kinase, is a member of the Ras MAPK pathway.
This pathway modulates the activity of transcription factors, structural proteins and other protein kinases, leading to biological responses such as cell proliferation and differentiation.
MEK2 contains a proline-rich segment that is recognized by the scaffold protein MEK binding partner-1 in vitro but not in vivo.
Similar to MEK1, MEK2 is phosphorylated by several kinases, including Raf1, c-Mos and MEKK1.
Activated MEK2 associates with and phosphorylates MAP kinases ERK1 and 2.
The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MAP2K2 when phosphorylated at Thr394. Species sequence homology: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to aa around the phosphorylation site of threonine 394 (P-G-TP-P-T) (KLH).
