Mammalian MD1 is a homolog of chicken MD1, which was previously isolated as a v-myb-regulated gene, since its transcription increases rapidly after v-myb induction.
MD1 is a 28kD molecule that is associated at the cell surface with RP105 (CD180).
RP105 is a leucine-rich repeat (LRR) molecule that is expressed on B lymphocytes.
It was first identified by a mAb that protects spleen B cells from irradiation-induced apoptosis.
LRR proteins, such as Toll receptors, have a role in innate immunity.
MD1 is primarily expressed by B cells, dendritic cells and monocytes, and promotes the recognition of, and subsequent signaling by LPS in the innate immune system.
MD1 does not have any homology to other molecules.
MD1 when expressed alone behaves as a secretory protein.
Expression of MD1 in a cell increases the expression of RP105.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human MDI. Species sequence homology: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 15aa from near the center of human MD-1.
