Matrix metalloproteinase-7 (MMP-7) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans.
MMP-7 is closely related to the stromelysin family members but is encoded by a different gene.
MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain.
It lacks the hemopexin-like domain common to other members of the MMPs.
MMP-7 is secreted as a 28kD proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18kD active MMP-7 enzyme.
Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2.
MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of gliomas.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlImmunoprecipitation: 2-5ng labels 10e6 cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity：Recognizes human MMP7. Species sequence homology: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the C-terminal of human MMP7 (KLH).
