LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor.
LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain.
This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase.
It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.
LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3.
This protein may play a role in the repression of neuronal genes.
Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity.
It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 5ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human KDM1A. Species sequence homology: Canine, macaque, monkey, mouse, rat and zebrafish.
