Kir6.2/Kcnj11, an ATP sensitive inwardly-rectifying potassium channel, was originally isolated from an insulinoma library.
Actual conductance requires association with the sulfonylurea receptor.
This receptor is inhibited by sulfonylureas and activated by diazoxide.
Mutations in the KNCJ11 gene are found in patients with permanent diabetes mellitus of infancy and hyperinsulinemic hypoglycemia.
It is thought that this channel couples metabolism to membrane electrical events fibringing about insulin secretion.
Applications:Suitable for use in Western Blot, ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:64,000Western Blot: 0.01-0.03ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 3.75ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human KCNJ11. Species sequence homology: Canine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to ERRARFVSKKGNC from an internal region of human KCNJ11.
