Interleukin-33 (IL-33), also known as NF-HEV and DVS 27, is a member of IL-1 cytokine family.
IL-33 is constitutively expressed in smooth muscle and airway epithelia and is upregulated by IL-1 Alpha or Beta in arterial smooth muscle, dermal fibroblasts, and keratinocytes.
IL-33 is synthesized as a 270aa, 32kD proform that localizes to the nucleus and may regulate gene transcription.
Cleavage generates mature IL-33 as an 18kD C-terminal proinflammatory fragment which binds IL-1 R4/ST2 on Th2 and mast cells.
Mature human IL-33 shares 55% aa identity with mouse IL-33.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry (Formalin-fixed, paraffin-embedded): 5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human IL33. Species sequence homology: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 19aa from near the center of human IL-33 (KLH).
