HOX11, also called homeobox11 or Tcl-3 is located at the 10q24 T-Cell translocation breakpoint region.
It encodes a homeobox-domain containing protein, HOX11, containing a glycine and proline-rich amino terminus.
HOX11 is required for maintenance of the developing spleen and cell survival.
The HOX11protein binds to a specific DNA sequence and localizes to the cell nucleus.
It transactivates transcription of a reporter gene linked to a cis-regulatory element, acting as a positive transcription activator.
The HOX11 gene is highly expressed in T-ALLs as a result of a t (7:10) (q35;q24) or t(10:14)(q24;qll) translocation.
The homeobox gene is deregulated upon translocation into the T-cell receptor locus and activates a specific subset of tumor-associated target genes.
HOX 11 gene translocation has been attributed to T-cell leukemia and lymphoid neoplasias.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human TLX1. Species sequence homology: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa300-330 of human HOX11 (KLH).
