EIF2 is a translation initiation factor that catalyzes the first regulated step of protein synthesis, promoting the binding of the initiator tRNA to 40s ribosomal subunits.
eIF2 is composed of 3 non identical subunits, alpha, beta and gamma.
Binding occurs as a ternary complex of methionyl-tRNA, eIF2 and GTP.
The rate of complex formation is modulated by the phosphorylation state of eIF2alpha.
Phosphorylation of eIF2alpha has been shown to occur in response to heat shock and hypoxic stress.
Once eIF2alpha is phosphorylated, the initiation of protein synthesis is inhibited.
Expression of eIF-2alpha has been shown to increase in melanoma and colon carcinoma.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human EIF2A. Species sequence homology: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic non-phosphopeptide corresponding to aa surrounding Ser 51 of human EIF2A (KLH).
