EIF-2alpha, also known as eukaryotic translation initiation factor 2alpha, promotes the binding of the initiator tRNA to the 40S ribosomal subunits and controls translational rates via phosphorylation/dephosphorylation mechanisms.
The double-stranded RNA (dsRNA)-activated protein kinase (PKR) provides an important control in the regulation of protein synthesis initiation through the phosphorylation of eIF-2alpha.
Phosphorylated eIF-2 alpha inhibits cell growth and induces apoptosis.
Phosphorylation of eIF-2alpha occurs under a variety of conditions including viral infection, apoptosis, nutrient deprivation, heme-deprivation, and certain stresses.
EIF-2 exists as a heterotrimeric protein composed of 3 nonidentical subunits, alpha (36kD), beta (38kD), and gamma (52kD).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes mouse EIF2A. Species sequence homology: human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant corresponding to mouse EIF2A at C-terminal.
