The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3).
Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family.
Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer.
ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas.
Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes human ERBB2. Species sequence homology: Mouse and rat.
Isotype：IgY
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa153-433 from human ERBB2.
