84-1205-71　［取扱停止］EPHA2 (Ephrin Type-A Receptor 2, Epithelial Cell Kinase, Tyrosine-protein Kinase Receptor ECK, ECK) 50ug　E3360-01M

特徴

• EphA2, also known as Eck, Myk2, and Sek2, is a member of the Eph receptor family which binds members of the ephrin ligand family.
• There are two classes of receptors, designated A and B.
• Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains.
• This is followed by the transmembrane region and cytoplasmic region.
• The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail, which contains one conserved tyrosine residue.
• Activation of kinase activity occurs after ligand recognition and binding.
• EphA2 has been shown to bind ephrin-A3, ephrin-A1, ephrin-A5, ephrin-A4, and ephrin-A2.
• The extracellular domains of mouse and human EphA2 share greater than 92% aa identity.
• Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro.
• While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation.
• In vivo, the ligands and receptors display reciprocal expression.
• It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue.
• The Eph/ephrin families also appear to play a role in angiogenesis.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.