Prostaglandin-endoperoxide synthase (fatty acid cyclooxygenase; PGH synthase; Cox-2) is a key enzyme in prostaglandin biosynthesis.
The first rate-limiting step in the conversion of arachidonic acid to prostaglandins is catalyzed by Cox-1 and Cox-2.
The cyclooxygenase activity of Cox-2 is inhibited by nonsteroidal antiinflammatory drugs such as aspirin and endomethacin.
It is a mitogen-inducible gene (unlike Cox-1, which is a house-keeping gene), and this means it probably is a primary mediator of the inflammatory response.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in TBS, pH 7.4, 0.1% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PTGS2. Species sequence homology: Mouse, rat and sheep.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa578-596 from human PTGS2 (KLH).
